Kusumastuti, Melati Yulia
(2012)
Micropropagation Of Curcuma Xanthorrhiza Roxb. And Zingiber Aromaticum Vahl. With Antimicrobial Activities Via Aerated Culture System.
Masters thesis, Universiti Sains Malaysia.
Abstract
The aseptic rhizomatous buds of Curcuma xanthorrhiza and Zingiber aromaticum were established by surface sterilizing with 70% ethanol for 20 minutes followed by 50% (v/v) Clorox® for 20 minutes. This procedure produced 100% and 83.3% aseptic buds explants of C. xamthorrhiza and Z. aromaticum respectively. All the aseptic buds of both species survived when cultured on MS medium supplemented with 0.5 mg/L BA and 0.5 mg/L IBA. The number of shoots formed from each shoot explant for both species using MS medium supplemented with 1.0 mg/L BA (C. xanthorrhiza 3..7 shoots per explant; Z. aromaticum 4.3 shoots per explant) was found to be not significantly different when compared with cultures using MS supplemented 0.5 mg/L BA and 0.5 mg/L IBA (C. xanthorrhiza 3.9 shoots per explant; Z. aromaticum 3.0 shoots per explant).
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