Othman, Afaf Alani Che
(2004)
Optimization of sugar assimilation test for identification of clinically isolated yeast.
Other thesis, Universiti Sains Malaysia.
Abstract
The study to developed the sugar assimilation test for identification of clinically isolated
yeast was done using the swab lawning method and the method of incorporation of
suspension on to medium. The result obtained, showed that the swab lawning method
was not feasible to be used for identifying clinically isolated yeast based on the sugar
assimilation test However, the method of incorporation of suspension on medium was
comparatively better and was carried out by optimizing the test by varying the sugar
concentration, medium pH and incubation temperature. Candida spp. were screened using
14 types of sugars. The optimized conditions for identification purpose was 20% ( w/v) of
sugar concentration, medium pH of 5.6 and the incubation temperature of 30°C for both
Candida albican and Candida tropicalis. Under these optimized conditions for
identification, Candida a/bican did not utilized cellobiose, whereas Candida tropica/is
utilized cellobiose. But the optimum growth conditions for both species were same.
Therefore the method of incorporation of suspension under the optimized conditions may
be used in the identification of clinically isolated yeast especially in differentiating
Candida a/bican and Candida tropical is.
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