Lew, Lee Ching
(2013)
Bioactives From Probiotic For Dermal
Health Upon Physical Treatments.
Masters thesis, Universiti Sains Malaysia.
Abstract
Lactobacilli and bifidobacteria are the most common genera of probiotics
with documented potentials on gut health. Recent studies suggested that such
potentials can be extended beyond gut well-being, such as that of dermal health. This
study was aimed to evaluate the production of bioactives that are essential for skin
health from either lactobacilli or bifidobacteria grown in milk. Lipoteichoic acid
content in the cell wall fraction was higher (P < 0.05) in Lactobacillus rhamnosus
FTDC 8313 and Bifidobacterium longum BL 8643 compared to the other strains
studied. Meanwhile, all strains studied showed equal concentration of cell wall
peptidoglycan. Our results showed that lactobacilli produced higher concentration of
hyaluronic acid and diacetyl than bifidobacteria. Strains of lactobacilli and
bifidobacteria studied also produced acid and neutral sphingomyelinase (SMase),
enzymes that generate ceramides which subsequently play important roles in
development of physical barriers in the stratum corneum (SC).
Sphingomyelinase activity in L. rhamnosus FTDC 8313 was also optimized
using divalent metal ions via response surface methodology. Using a full-factorial
design for factors screening, only magnesium sulphate (MgSO4) and manganese
sulphate (MnSO4) significantly (P < 0.001) influenced SMase activity while the
effects of calcium sulphate (CaSO4) was insignificant (P > 0.05).
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