The study of glucose-6-phosphate dehydrogenase (G6PD) gene by restriction enzyme by restriction enzyme digestion in the Kelantan population

Yusoff, Narazah Mohd and Jamaluddin, Normah and Hussin, Zabidi Azhar Mohd and Ee, Choo Keng and Sein, Kyaw Tun The study of glucose-6-phosphate dehydrogenase (G6PD) gene by restriction enzyme by restriction enzyme digestion in the Kelantan population. In: The study of glucose-6- phosphate dehydrogenase (G6PD) gene by restriction enzyme by restriction enzyme digestion in the Kelantan population. (Submitted)

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Abstract

The most common diseases producing enzymopathy affecting the human population is glucose-6-phosphate dehydrogenase (G6PD) deficiency. It is estimated that about 400 million people worldwide are affected and this disease is commonest seen in the tropical and subtropical zones of the Eastern hemisphere. Molecular analysis has confirmed that the basis for G6PD deficiency is widely heterogeneous. Different mutants are responsible for the G6PD deficiency in the various parts of the world where this abnormality is prevalent. This study involved a sequential analysis whereby the blood from Malay neonates with neonatal jaundice admitted to Hospital Universiti Sains Malaysia and Kota Bharu Hospital were analyzed and polymerase chain reaction based analysis using serial multiplex primer method was done on those DNA samples. Samples that are found to be abnormal were then sequenced. Out of the 45 samples studied, 8 were found to have the Mediterranean mutation, two have the Mahidol mutation, two have Canton mutation and three have Kaiping mutation. Thus the molecular basis for the Malay neonatal jaundice in Kelantan is described with further prospect of population screening.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Glucose-6-phosphate dehydrogenase, enzyme, polymerase chain reaction
Subjects: R Medicine > R Medicine (General) > R5-920 Medicine (General)
Divisions: Kampus Kesihatan (Health Campus) > Pusat Pengajian Sains Perubatan (School of Medical Sciences) > Conference or Workshop Item
Depositing User: Mr Abdul Hadi Mohammad
Date Deposited: 23 Mar 2020 05:04
Last Modified: 23 Mar 2020 05:04
URI: http://eprints.usm.my/id/eprint/42642

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