The Cyto-Genotoxicity Evaluation Of Biomedical Grade Chitosan On Primary Keratinocyte Cultures And Its Role In Scar Formation Pathways Using Skin Coculture Model

Lim , Chin Keong (2010) The Cyto-Genotoxicity Evaluation Of Biomedical Grade Chitosan On Primary Keratinocyte Cultures And Its Role In Scar Formation Pathways Using Skin Coculture Model. PhD thesis, Universiti Sains Malaysia.

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Abstract

Chitosan (-1, 4—D-glucosamine) is a deacetylated form of chitin with excellent biological properties useful in wound management. However, improvement of the physical and chemical properties of the chitosan will alter its biocompatibility. Additionally, chitosan has been reported to be useful in scar intervention. Therefore in this study, the in vitro biocompatibility of the modified chitosan porous skin regenerating templates (CPSRTs) has been evaluated based on cytotoxicity analysis by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay, genotoxicity by comet assay, and skin keratinocyte pro-inflammatory cytokine secretions [interleukin-1 (IL-1), tumor necrosis factor- (TNF-) and interleukin-8 (IL-8)], using primary normal human epidermal keratinocyte (pNHEK) cultures. The most biocompatible CPSRT was then investigated for its role in keloid intervention using keratinocyte-fibroblast cocultures. The human collagen type-I, III and V protein expressions were assayed in Western blot analysis.

Item Type: Thesis (PhD)
Subjects: R Medicine > R Medicine (General) > R5-920 Medicine (General)
Divisions: Kampus Kesihatan (Health Campus) > Pusat Pengajian Sains Perubatan (School of Medical Sciences) > Thesis
Depositing User: Mr Mohammad Harish Sabri
Date Deposited: 04 Oct 2018 03:07
Last Modified: 12 Apr 2019 05:26
URI: http://eprints.usm.my/id/eprint/42288

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