Abraham, Fariz
(2010)
Elicitation Effect On The Growth And Biochemical Activities Of Curcuma Mangga Val (Zingiberaceae) In Vitro Plantlets.
Masters thesis, Universiti Sains Malaysia.
Abstract
Curcuma mangga aseptic plantlets could be established via double-stage surface sterilization technique. The bud explants derived from the rhizomes were immersed in 70% (v/v) alcohol for ten minutes at the first stage followed by surface-sterilized with 20% (v/v) Clorox® for 20 minutes at the second stage. The established aseptic explants were then cultured on the best proliferation medium, which is the MS medium supplemented with 2 mgL-1 6-benzylaminopurine (BAP) and 0.5 mgL-1 1-naphthaleneacetic acid (NAA). Liquid proliferation medium induced more auxiliary shoots (6.7 ± 0.4 shoots/explant) as compared to gelled proliferation medium (4.6 ± 0.6 shoots/explant).
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