Pengoptimuman Penghasilan Dan Penulenan Enzim Keratinase Daripada Microsporum Gypseum S(F)23 Pencilan Tempatan Menggunakan Fermentasi Kultur Tenggelam

Kamal, Syazni Zainul (2009) Pengoptimuman Penghasilan Dan Penulenan Enzim Keratinase Daripada Microsporum Gypseum S(F)23 Pencilan Tempatan Menggunakan Fermentasi Kultur Tenggelam. Masters thesis, Universiti Sains Malaysia.

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Abstract

Bulu ayam yang mengandungi 90% keratin merupakan bahan buangan industri ternakan yang dihasilkan dalam kuantiti yang besar setiap tahun. Oleh itu, penyelidikan ini dirangka bagi menghasilkan enzim keratinase yang berupaya menghidrolisis keratin bulu ayam kepada protein serta asid amino yang berpotensi digunakan di dalam makanan ternakan. Pencilan yang digunakan dalam penghasilan enzim keratinase dicamkan sebagai Microsporum gypseum. Pencilan ini telah dipencilkan melalui kaedah pengumpanan substrat keratin dari sampel tanah kawasan kandang kuda di Georgetown, Pulau Pinang. Microsporum gypseum S(F)23 menghasilkan enzim keratinase secara ekstrasel dan mencapai aktiviti maksimum selepas 6 hari pengkulturan di dalam medium asas keratin. Chicken feathers which consist 90% keratin are produce as a waste by poultry processing industries in huge quantities every year. So, this research was developed to produce keratinase that are able to degrade keratin into protein and amino acid to be use in poultry dietary. The isolate that was used in the production of keratinase was identified as Microsporum gypseum. The isolate was isolated using a baiting technique from soil sample from a horse stable located in Georgetown, Pulau Pinang. Microsporum gypseum S(F)23 achieved maximum extracellular keratinase activity after 6 days of incubation.

Item Type: Thesis (Masters)
Subjects: Q Science > QH Natural history > QH1 Natural history (General - Including nature conservation, geographical distribution)
Divisions: Pusat Pengajian Sains Kajihayat (School of Biological Sciences) > Thesis
Depositing User: Mr Mohammad Harish Sabri
Date Deposited: 17 Aug 2018 07:48
Last Modified: 12 Apr 2019 05:27
URI: http://eprints.usm.my/id/eprint/41378

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