Development of Multiplexed Infectious Disease Lateral Flow Assays: Challenges and Opportunities

Hanafiah, Khayriyyah Mohd and Arifin, Norsyahida and Bustami, Yazmin and Noordin, Rahmah and Garcia, Mary and Anderson, David (2017) Development of Multiplexed Infectious Disease Lateral Flow Assays: Challenges and Opportunities. Diagnostics, 7 (3). p. 51. ISSN 2075-4418

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Abstract

Lateral flow assays (LFAs) are the mainstay of rapid point-of-care diagnostics, with the potential to enable early case management and transform the epidemiology of infectious disease. However, most LFAs only detect single biomarkers. Recognizing the complex nature of human disease, overlapping symptoms and states of co-infections, there is increasing demand for multiplexed systems that can detect multiple biomarkers simultaneously. Due to innate limitations in the design of traditional membrane-based LFAs, multiplexing is arguably limited to a small number of biomarkers. Here, we summarize the need for multiplexed LFA, key technical and operational challenges for multiplexing, inherent in the design and production of multiplexed LFAs, as well as emerging enabling technologies that may be able to address these challenges. We further identify important areas for research in efforts towards developing multiplexed LFAs for more impactful diagnosis of infectious diseases.

Item Type:	Article
Uncontrolled Keywords:	lateral flow assay; point-of-care; multiplex; diagnostic test; infectious disease; resource-limited settings
Subjects:	Q Science > QH Natural history > QH301 Biology
Divisions:	Pusat Pengajian Sains Kajihayat (School of Biological Sciences) > Article Zoom Profil Pakar (Expert Profile) > Siti Khayriyyah Mohd Hanafiah (Biological Sciences)
Depositing User:	Administrator Automasi
Date Deposited:	03 Jul 2018 05:19
Last Modified:	03 Jul 2018 05:19
URI:	http://eprints.usm.my/id/eprint/40878

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