Xanthorrhizol Induces Apoptosis Via the Up-regulation of Bax and p53 in HeLa Cells

Ismail, Norzila and Hawariah, Azimahtol and Pihie, Lope and Nallapan, Meenakshii (2005) Xanthorrhizol Induces Apoptosis Via the Up-regulation of Bax and p53 in HeLa Cells. Anticancer Research, 25 (3B). pp. 2221-2228. ISSN 0250-7005

[img]
Preview
 PDF
Download (252kB) | Preview

Abstract

Xanthorrhizol is a sesquiterpenoid compound extracted from Curcuma xanthorrhiza, which is known locally as Temulawak. Traditionally, C. xanthorrhiza was found to have antibacterial, anticancer and anti-inflammatory activity. The rhizome has also been used to treat inflammation in postpartum uterine bleeding. An antiproliferative assay using methylene blue staining revealed that xanthorrhizol inhibited the proliferation of the cervical cancer cell line HeLa with an EC50 value of 6.16 Ìg/ml. Xanthorrhizol significantly increased apoptosis in HeLa cells, as evaluated by the Tdt-mediated dUTP nick end-labelling (TUNEL) assay and nuclear morphology by Hoechst 33258 staining. Western blot analysis, which was further confirmed by the immunostaining results, implied an up-regulation of tumor suppressor protein p53 and the pro-apoptotic protein Bax, following the treatment with xanthorrhizol. Xanthorrhizol, however, did not affect the expression of the anti-apoptotic protein, Bcl-2 and the viral oncoprotein, E6. Hence, xanthorrhizol is a promising antiproliferative and anticancer agent which induces p53 and Bax-dependent apoptosis in HeLa cervical cancer cells.

Item Type: Article
Subjects: R Medicine > RA Public aspects of medicine > RA643-645 Disease (Communicable and noninfectious) and public health
Divisions: Kampus Kesihatan (Health Campus) > Pusat Pengajian Sains Perubatan (School of Medical Sciences) > Article
Zoom Profil Pakar (Expert Profile) > Norzila Ismail (Medical Sciences)
Depositing User: Administrator Automasi
Date Deposited: 07 Jun 2018 01:11
Last Modified: 07 Mar 2019 01:43
URI: http://eprints.usm.my/id/eprint/40710

Actions (login required)

View Item View Item
USM Repository is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.
Share