Entamoeba histolytica acetyl-CoA synthetase: biomarker of acute amoebic liver abscess

Lim, Boon Huat and Alfonso, Olivos Garcia and Tan, Zi Ning and Wong, Weng Kin and Noordin, Rahmah and Anaqi Azham, Siti Shafiqah and Lee, Zhi Jie and Guee, Cher Ching and Foo, Phiaw Chong and Pim, Chau Dam (2014) Entamoeba histolytica acetyl-CoA synthetase: biomarker of acute amoebic liver abscess. Asian Pacific Journal of Tropical Biomedicine, 4 (6). pp. 446-450. ISSN 2221-1691

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Abstract

Objective: To characterize the Entamoeba histolytica (E. histolytica) antigen(moribound amoebic liver abscess hamsters. s) recognized by Methods: Crude soluble antigen of E. histolytica was probed with sera of moribund hamsters isnp e1cDtr-o manedtr y2 Da-naWlyessitse.r nT hbel octo arrneaslpyosnesd.i nTgh eg eannet iwgeansi cc lopnroetde iann wd aesx pthreenss esden itn f oErs ctahnerdiecmhi am caoslsi BprLo2t1e-inA.I to produce the recombinant E. histolytica ADP-forming acetyl-CoA synthetase (EhACS) recombinAa nctu psrtootmeiins.ed ELISA was developed to evaluate the sensitivity and specificity of the Results: A ~75 kDa protein band with a pI value of 5.91-6.5 was found to be antigenic; and not dtheete pctreodte biny tsoe rbae o tfh hea 7m7s tkeDrsa iEn . thheis tcoolnyttricoal gAroDuPp-. fToramndinegm amceatsysl -spCeocAt rsoymnetthreyt aasnea l(yEshisA rCeSv)e. aTlhede icnufsetcotmedi s(end= 3E1L) aISnAd croensutrlotsl grreovuepa lheadm 1s0te0r%s (sne=n5s)i stievriutym asnadm p10le0s%, rsepsepceicftiicvietyly .w hen tested against Conclusions: This finding suggested the significant role of EhACS as a biomarker for moribund shtaumdisetse resx pwliothre athcue tpeo atemnotieabl irco lleivs eorf aEbhsAcCeSss i n(A bLeAtt)e rin ufnecdteirosnta. nItd iinsg d teheem peadth pogeertnineseinst o tfh AaLt Afu; taunrde in the development of vaccine and diagnostic tests to control ALA in human populations.

Item Type: Article
Subjects: R Medicine > R Medicine (General) > R735-854 Medical education. Medical schools. Research
Divisions: Zoom Profil Pakar (Expert Profile) > Professor Dr. Rahmah Noordin (Institute for Research In Molecular Medicine)
Institut Penyelidikan Perubatan Molekul (Institute for Research in Molecular Medicine INFORMM) > Article
Kampus Kesihatan (Health Campus) > Pusat Pengajian Sains Kesihatan (School of Health Sciences) > Article
Depositing User: Mr Noorazilan Noordin
Date Deposited: 15 Feb 2018 07:35
Last Modified: 15 Feb 2018 07:35
URI: http://eprints.usm.my/id/eprint/38975

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