Effects of Droplet-Vitrification Cryopreservation Based on Physiological and Antioxidant Enzyme Activities of Brassidium Shooting Star Orchid

Rahmah, Safrina and Ahmad Mubbarakh, Safiah and Khor, Soo Ping and Subramaniam, Sreeramanan (2015) Effects of Droplet-Vitrification Cryopreservation Based on Physiological and Antioxidant Enzyme Activities of Brassidium Shooting Star Orchid. Scientific World Journal, 2015 (961793). pp. 1-10. ISSN 2356-6140

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Abstract

Protocorm-like bodies (PLBs) of Brassidium Shooting Star orchid were successfully cryopreserved using droplet-vitrification method. Vitrification based cryopreservation protocol is comprised of preculture, osmoprotection, cryoprotection, cooling, rewarming, and growth recovery and each and every step contributes to the achievement of successful cryopreservation. In order to reveal the lethal and nonlethal damage produced by cryopreservation, histological observation, scanning electron microscopy (SEM), and biochemical analysis were carried out in both cryopreserved and noncryopreserved PLBs of Brassidium Shooting Star orchid comparing with the control PLBs stock culture. Histological and scanning electron microscopy analyses displayed structural changes in cryopreserved PLBs due to the impact of cryoinjury during exposure to liquid nitrogen. Total soluble protein significantly increased throughout the dehydration process and the highest value was achieved when PLBs were stored in liquid nitrogen. Ascorbate peroxidase (APX) and catalase (CAT) showed the highest enzyme activities in both dehydration and cryostorage treatments indicating that stress level of PLBs was high during these stages.

Item Type: Article
Subjects: Q Science > QH Natural history > QH1 Natural history (General - Including nature conservation, geographical distribution)
Divisions: Pusat Pengajian Sains Kajihayat (School of Biological Sciences) > Article
Zoom Profil Pakar (Expert Profile) > Sreeramanan A/L Subramaniam (Biological Sciences)
Depositing User: Mr Noorazilan Noordin
Date Deposited: 14 Feb 2018 00:28
Last Modified: 14 Feb 2018 00:28
URI: http://eprints.usm.my/id/eprint/38896

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