Decreased expression of alpha-2-HS glycoprotein in the sera of rats treated with Eurycoma longifolia extract

Yeng, Chen and Phang, Wai Mei and Mu, Alan K.W. and Chan, Choon Keat and Low, Bin Seng and Sasidharan, Sreenivasan and Chan, Kit Lam (2015) Decreased expression of alpha-2-HS glycoprotein in the sera of rats treated with Eurycoma longifolia extract. Frontiers in Pharmacology, 6 (211). pp. 1-6. ISSN 1663-9812

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Abstract

Eurycoma longifolia is a Malaysian native herb that has been widely used as an aphrodisiac and a remedy for andropause. Although the physiological effects of the plant extract were predicted as a result of the alterations in protein expression, the key protein(s) involved in these alterations are still unclear. In the present study, we have investigated the effect of standardized E. longifolia extract on serum protein expression up to 28 days following oral administration in rats. Serum protein profiles were analyzed by 2-dimensional electrophoresis, and altered proteins were identified via mass spectrometry. We observed that alpha-2-HS glycoprotein (AHS) was significantly decreased in the serum of experimentally treated rats compared to pre-treated animals. Moreover, reduction in AHS was confirmed using competitive enzyme-linked immunosorbent assay. AHS expression is known to be associated with insulin resistance and diabetes. Our data indicated that serum AHS was reduced in rats treated with standardized E. longifolia extract, and therefore form a prelude for further investigation into the effects of this natural extract in animal models involving infertility and diabetes.

Item Type: Article
Subjects: R Medicine > R Medicine (General) > R735-854 Medical education. Medical schools. Research
R Medicine > RS Pharmacy and materia medica > RS1-441 Pharmacy and materia medica
Divisions: Institut Penyelidikan Perubatan Molekul (Institute for Research in Molecular Medicine INFORMM) > Article
Pusat Pengajian Sains Farmasi (School of Pharmacy) > Article
Zoom Profil Pakar (Expert Profile) > Associate Professor Dr. Sasidharan A/L Sreenivasan (Institute for Research In Molecular Medicine)
Depositing User: Mr Noorazilan Noordin
Date Deposited: 01 Feb 2018 01:04
Last Modified: 01 Feb 2018 01:04
URI: http://eprints.usm.my/id/eprint/38617

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