Ooi, Kheng Leong and Tengku Muhammad, Tengku Sifzizul and Sulaiman, Shaida Fariza
(2011)
Cytotoxic Activities of Physalis minima L. Chloroform
Extract on Human Lung Adenocarcinoma NCI-H23 Cell
Lines by Induction of Apoptosis.
Evidence-Based Complementary and Alternative Medicine, 2011 (185064).
pp. 1-10.
ISSN 1741-427X
Abstract
Physalis minima L. is reputed for having anticancer property. In this study, the chloroformextract of this plant exhibited remarkable
cytotoxic activities on NCI-H23 (human lung adenocarcinoma) cell line at dose- and time-dependent manners (after 24, 48 and
72 h of incubation). Analysis of cell-death mechanism demonstrated that the extract exerted apoptotic programed cell death
in NCI-H23 cells with typical DNA fragmentation, which is a biochemical hallmark of apoptosis. Morphological observation
using transmission electron microscope (TEM) also displayed apoptotic characteristics in the treated cells, including clumping
and margination of chromatins, followed by convolution of the nuclear and budding of the cells to produce membrane-bound
apoptotic bodies. Different stages of apoptotic programed cell death as well as phosphatidylserine externalization were confirmed
using annexin V and propidium iodide staining. Furthermore, acute exposure to the extract produced a significant regulation of
c-myc, caspase-3 and p53mRNA expression in this cell line. Due to its apoptotic effect on NCI-H23 cells, it is strongly suggested
that the extract could be further developed as an anticancer drug.
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