Conventional Rapid Latex Agglutination in Estimation of von Willebrand Factor: Method Revisited and Potential Clinical Applications

Mahat, Marianor and Abdullah, Wan Zaidah and Che Hussin, Che Maraina (2014) Conventional Rapid Latex Agglutination in Estimation of von Willebrand Factor: Method Revisited and Potential Clinical Applications. Journal of Immunology Research, 2014 (850810). pp. 1-10. ISSN 2314-8861

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Abstract

Measurement of von Willebrand factor antigen (VWF:Ag) levels is usually performed in a specialised laboratory which limits its application in routine clinical practice. So far, no commercial rapid test kit is available for VWF:Ag estimation. This paper discusses the technical aspect of latex agglutinationmethod which was established to suit the purpose of estimating vonWillebrand factor (VWF) levels in the plasma sample. The latex agglutination test can be performed qualitatively and semiquantitatively. Reproducibility, stability, linearity, limit of detection, interference, and method comparison studies were conducted to evaluate the performance of this test. Semiquantitative latex agglutination test was strongly correlated with the reference immunoturbidimetric assay (Spearman’s rho = 0.946,

Item Type:	Article
Subjects:	Q Science > QR Microbiology > QR180-189.5 Immunology
Divisions:	Kampus Kesihatan (Health Campus) > Pusat Pengajian Sains Perubatan (School of Medical Sciences) > Article
Depositing User:	Mr Noorazilan Noordin
Date Deposited:	24 Jan 2018 06:12
Last Modified:	24 Jan 2018 06:12
URI:	http://eprints.usm.my/id/eprint/38530

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