Yahaya, B. and McLachlan, G. and Collie, D. D. S.
(2013)
BrdU Pulse Labelling In Vivo to Characterise Cell Proliferation during Regeneration and Repair following Injury to the Airway Wall in Sheep.
Scientific World Journal, 2013 (871932).
pp. 1-5.
ISSN 2356-6140
Abstract
The response of S-phase cells labelled with bromodeoxyuridine (BrdU) in sheep airways undergoing repair in response to
endobronchial brush biopsy was investigated in this study. Separate sites within the airway tree of anaesthetised sheep were biopsied
at intervals prior to pulse labelling with BrdU, which was administered one hour prior to euthanasia. Both brushed and spatially
disparate unbrushed (control) sites were carefully mapped, dissected, and processed to facilitate histological analysis of BrdU
labelling. Our study indicated that the number and location of BrdU-labelled cells varied according to the age of the repairing
injury. There was little evidence of cell proliferation in either control airway tissues or airway tissues examined six hours after
injury. However, by days 1 and 3, BrdU-labelled cells were increased in number in the airway wall, both at the damaged site and
in the regions flanking either side of the injury. Thereafter, cell proliferative activity largely declined by day 7 after injury, when
consistent evidence of remodelling in the airway wall could be appreciated. This study successfully demonstrated the effectiveness
of in vivo pulse labelling in tracking cell proliferation during repair which has a potential value in exploring the therapeutic utility
of stem cell approaches in relevant lung disease models.
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