Antioxidant and Toxicity Studies of 50% Methanolic Extract of Orthosiphon stamineus Benth

Mun, Fei Yam and Chung, Pin Lim and Lee, Fung Ang and Lip, Yee Por and Siew, Tung Wong and Asmawi, Mohd. Zaini and Basir, Rusliza and Ahmad, Mariam (2013) Antioxidant and Toxicity Studies of 50% Methanolic Extract of Orthosiphon stamineus Benth. BioMed Research International, 2013 (351602). pp. 1-10. ISSN 2314-6133

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Abstract

The present study evaluated the antioxidant activity and potential toxicity of 50% methanolic extract of Orthosiphon stamineus (Lamiaceae) leaves (MEOS) after acute and subchronic administration in rats. Superoxide radical scavenging, hydroxyl radical scavenging, and ferrous ion chelating methods were used to evaluate the antioxidant properties of the extract. In acute toxicity study, single dose of MEOS, 5000mg/kg, was administered to rats by oral gavage, and the treated rats were monitored for 14 days. While in the subchronic toxicity study, MEOS was administered orally, at doses of 1250, 2500, and 5000mg/kg/day for 28 days. From the results, MEOS showed good superoxide radical scavenging, hydroxyl radical scavenging, ferrous ion chelating, and antilipid peroxidation activities. There was no mortality detected or any signs of toxicity in acute and subchronic toxicity studies. Furthermore, there was no significant difference in bodyweight, relative organ weight, and haematological and biochemical parameters between bothmale and female treated rats in any doses tested.No abnormality of internal organs was observed between treatment and control groups.Theoral lethal dose determined wasmore than 5000mg/kg and the no-observed-adverse-effect level (NOAEL) of MEOS for both male and female rats is considered to be 5000mg/kg per day.

Item Type: Article
Subjects: R Medicine > RS Pharmacy and materia medica > RS1-441 Pharmacy and materia medica
Divisions: Pusat Pengajian Sains Farmasi (School of Pharmacy) > Article
Depositing User: Mr Noorazilan Noordin
Date Deposited: 11 Jan 2018 02:07
Last Modified: 11 Jan 2018 02:07
URI: http://eprints.usm.my/id/eprint/38338

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