Preliminary comparative analysis of antibacterial effects of activated and non-activated of expired platelet concentrate by disc diffusion method

Zabidi, Muhammad Azrul and Mohd Yusoff, Narazah and Sheik Abdul Kader, Zainoodin (2012) Preliminary comparative analysis of antibacterial effects of activated and non-activated of expired platelet concentrate by disc diffusion method. Indian Journal of Pathology and Microbiology, 55 (1). pp. 47-51. ISSN 0377-4929

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Abstract

Background: Platelets release more than 30 cytokines to provide primary hemostatic function. In addition, platelets are also known to release antimicrobial peptides upon activation by thrombin. Materials and Methods: In this study, comparative analysis of antibacterial activity of activated and non-activated expired platelet concentrate was determined against Grampositive and Gram-negative bacteria by Kirby-Bauer disk diffusion method. Thrombin was used to prepare activated platelet concentrate. Gram-positive bacteria tested in this study were S.aureus and S.pyogenes and Gram-negative bacteria were E.coli and K.oxytoca. All the bacteria used in this study were sensitive strains from clinical isolates. Activated and nonactivated platelet showed no zone of inhibition against S.pyogenes and E.coli. Results: Activated platelet showed antibacterial activity against S.aureus and K.oxytoca with the zone of inhibition of 8.3 ± 0.6 mm and 7.7 ± 0.2 mm, respectively. Zone of inhibition observed in non-activated platelet against S.aureus and K.oxytoca were 7.8 ± 0.4 mm and 7.5 ± 0.3 mm, respectively. Conclusions: These findings showed that no significant differences in antibacterial activity produced by activated and non-activated platelet. However, zone of inhibition observed in activated and non-activated platelet indicate the presence of antibacterial property in expired platelet.

Item Type:	Article
Subjects:	Q Science > QR Microbiology > QR1-502 Microbiology
Divisions:	Institut Perubatan & Pergigian Termaju (Advanced Medical & Dental Institute (AMDI)) > Article Zoom Profil Pakar (Expert Profile) > Muhammad Azrul Zabidi (Advanced Medical & Dental Institute) Zoom Profil Pakar (Expert Profile) > Narazah Mohd Yusoff (Advanced Medical & Dental Institute)
Depositing User:	Administrator Automasi
Date Deposited:	21 Nov 2017 03:11
Last Modified:	21 Nov 2017 03:11
URI:	http://eprints.usm.my/id/eprint/37573

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