Wai, Yean Leong and Chin, Fhong Soon and Soon, Chuan Wong and Kian, Sek Tee and Sok, Ching Cheong and Siew, Hua Gan and Youseffi, Mansour
(2017)
In Vitro Growth of Human Keratinocytes and Oral
Cancer Cells into Microtissues: An Aerosol-Based
Microencapsulation Technique.
Bioengineering, 4 (43).
pp. 1-14.
ISSN 2306-5354
Abstract
Cells encapsulation is a micro-technology widely applied in cell and tissue research, tissue
transplantation, and regenerative medicine. In this paper, we proposed a growth of microtissue model
for the human keratinocytes (HaCaT) cell line and an oral squamous cell carcinoma (OSCC) cell line
(ORL-48) based on a simple aerosol microencapsulation technique. At an extrusion rate of 20 �L/min
and air flow rate of 0.3 L/min programmed in the aerosol system, HaCaT and ORL-48 cells in alginate
microcapsules were encapsulated in microcapsules with a diameter ranging from 200 to 300 �m.
Both cell lines were successfully grown into microtissues in the microcapsules of alginate within
16 days of culture. The microtissues were characterized by using a live/dead cell viability assay,
field emission-scanning electron microscopy (FE-SEM), fluorescence staining, and cell re-plating
experiments. The microtissues of both cell types were viable after being extracted from the alginate
membrane using alginate lyase. However, the microtissues of HaCaT and ORL-48 demonstrated
differences in both nucleus size and morphology. The microtissues with re-associated cells in
spheroids are potentially useful as a cell model for pharmacological studies.
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