In Vitro Growth of Human Keratinocytes and Oral Cancer Cells into Microtissues: An Aerosol-Based Microencapsulation Technique

Wai, Yean Leong and Chin, Fhong Soon and Soon, Chuan Wong and Kian, Sek Tee and Sok, Ching Cheong and Siew, Hua Gan and Youseffi, Mansour (2017) In Vitro Growth of Human Keratinocytes and Oral Cancer Cells into Microtissues: An Aerosol-Based Microencapsulation Technique. Bioengineering, 4 (43). pp. 1-14. ISSN 2306-5354

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    Abstract

    Cells encapsulation is a micro-technology widely applied in cell and tissue research, tissue transplantation, and regenerative medicine. In this paper, we proposed a growth of microtissue model for the human keratinocytes (HaCaT) cell line and an oral squamous cell carcinoma (OSCC) cell line (ORL-48) based on a simple aerosol microencapsulation technique. At an extrusion rate of 20 �L/min and air flow rate of 0.3 L/min programmed in the aerosol system, HaCaT and ORL-48 cells in alginate microcapsules were encapsulated in microcapsules with a diameter ranging from 200 to 300 �m. Both cell lines were successfully grown into microtissues in the microcapsules of alginate within 16 days of culture. The microtissues were characterized by using a live/dead cell viability assay, field emission-scanning electron microscopy (FE-SEM), fluorescence staining, and cell re-plating experiments. The microtissues of both cell types were viable after being extracted from the alginate membrane using alginate lyase. However, the microtissues of HaCaT and ORL-48 demonstrated differences in both nucleus size and morphology. The microtissues with re-associated cells in spheroids are potentially useful as a cell model for pharmacological studies.

    Item Type: Article
    Subjects: R Medicine > R Medicine (General) > R5-130.5 General works
    Divisions: Kampus Kesihatan (Health Campus) > Pusat Pengajian Sains Perubatan (School of Medical Sciences) > Article
    Depositing User: Mr Noorazilan Noordin
    Date Deposited: 10 Oct 2017 09:17
    Last Modified: 10 Oct 2017 09:17
    URI: http://eprints.usm.my/id/eprint/36986

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