Perumal, Shanmugapriya and Yeng, Chen and Kanwar, Jagat Rakesh and Sasidharan, Sreenivasan (2016) Effects of Calophyllum inophyllum fruit extract on the proliferation and morphological characteristics of human breast cancer cells MCF-7. Asian Pacific Journal of Tropical Disease, 6 (4). pp. 291-297. ISSN 2222-1808
Full text not available from this repository.Abstract
Objective: To evaluate the antiproliferative activity of Calophyllum inophyllum (C. inophyllum) fruit extract against human breast cancer cells MCF-7. Methods: The cytotoxic effect of C. inophyllum fruit extract against MCF-7 cancer cells was evaluated through MTT and CyQuant assays for 24 h and the morphological investigation of treated MCF-7 cells was observed under optical microscope using Giemsa staining. Results: The cytotoxic effect of C. inophyllum fruit extract against MCF-7 cancer cells was evaluated through MTT and CyQuant assays simultaneously for 24 h after treatment, which demonstrated the inhibition of cell viability with the IC50 values of 19.63 μg/mL and 27.54 μg/mL, respectively. The preliminary time-based morphological investigation of MCF-7 cells treated with the IC50 value (23.59 μg/mL) of C. inophyllum fruit extract was observed under an optical microscopy via Giemsa staining, which exhibited prominent histological characteristics of apoptosis. Conclusions: This study clearly proved that the proliferation of human breast cancer cell MCF-7 was inhibited by C. inophyllum fruit extract resulted from the induction of apoptosis in MCF-7 cells.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) > R735-854 Medical education. Medical schools. Research |
| Divisions: | Institut Penyelidikan Perubatan Molekul (Institute for Research in Molecular Medicine INFORMM) > Article |
| Depositing User: | Mr Noorazilan Noordin |
| Date Deposited: | 04 Oct 2017 01:39 |
| Last Modified: | 04 Oct 2017 01:39 |
| URI: | http://eprints.usm.my/id/eprint/36912 |
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