Identification of potential protein biomarkers from salmonella enterica subspecies enterica serovar typhi biofilm

Lee, Yen Lurk (2013) Identification of potential protein biomarkers from salmonella enterica subspecies enterica serovar typhi biofilm. Masters thesis, Universiti Sains Malaysia.

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    Abstract

    Salmonella Typhi adalah patogen demam kepialu yang hanya menjangkiti manusia. Ia merupakan masalah kesihatan yang serius di negara-negara yang sedang membangun termasuk Malaysia. Walaupun langkah pencegahan dan rawatan perubatan telah dijalankan, tetapi wabak demam kepialu masih berlaku. Salah satu faktor yang menyumbang kepada keterusan deman kepialu adalah kewujudan pembawa (carrier) deman kepialu yang tiada tanda-tanda klinikal. Wujudnya pembawa deman kepialu adalah dijangka melibatkan biofilem yang terbentuk di dalam pudi hempedu pesakit. Pembentukan biofilem membolehkan bakteria tersebut mengelak serangan sistem imun mangsanya dan ubat antibiotik. Dalam usaha untuk memahami pembentukan biofilem, satu kaedah untuk kultur S. Typhi biofilem in vitro telah dicipta untuk memudahcarakan kajian ekpresi protein dan kekebalan biofilem terhadap antibiotik. Salmonella Typhi is a human specific pathogen which causes typhoid fever. It is a major health problem in developing countries including Malaysia. Although prevention and medical treatment are available, typhoid still persists. One of the major factors contributing to typhoid persistence is the existence of asymptomatic typhoid carriers. Biofilm formation in the human gallbladder is postulated to be associated with development of carriers. Formation of biofilm enables the bacteria to evade the host immune system and resist antibiotics. In order to understand the formation of biofilm, a S. Typhi biofilm culture method was developed to facilitate the biofilm protein expression and antibiotic resistance studies.

    Item Type: Thesis (Masters)
    Subjects: R Medicine > R Medicine (General) > R735-854 Medical education. Medical schools. Research
    Divisions: Kampus Kesihatan (Health Campus) > Pusat Pengajian Sains Perubatan (School of Medical Sciences)
    Depositing User: Mr Noorazilan Noordin
    Date Deposited: 08 Sep 2017 09:31
    Last Modified: 08 Sep 2017 09:31
    URI: http://eprints.usm.my/id/eprint/36416

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