Cloning and expression of CYP2D6*1 and CYP2D6*10 and its application in vitro drug-herbs interaction studies

Lee, Wee Leng (2013) Cloning and expression of CYP2D6*1 and CYP2D6*10 and its application in vitro drug-herbs interaction studies. PhD thesis, Universiti Sains Malaysia.

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Abstract

CYP2D6 mempakan salah satu enzim CYP utama dania bertanggungjawab dalam metabolisma kira-kira 20% daripada ubat-ubatan yang terdapat pada masa kini. CY2D6 adalah berpolymorfisma dan CYP2D6* 10 mempakan alelle kebiasaan di kalangan populasi Malaysia. Dat1. berkenaan interaksi herbal tempatan-ubatan adalah kekurangan. Objektif penyelidikan ini adalah menghasilkan assei in vitro berkenaan interaski ubatan-herbal dan mengunakannya untuk menganalisasi interaksi herbal tempatan. CYP2D6*1 rekombinan telah dihasilkan dan diekpreskan bersama CYP-reductase dalam bacteria E. coli. CYP2D6* 10 dihasilkan dengan kaedah "sitedirected mutagenesis" dan diekpreskan sebagai CYP2D6* 1. CYP2D6 is one of the maJor CYPs enzymes and is responsible for the metabolism of about 20% of cunently available therapeutic chugs. CYP2D6 is polymorphic and CYP2D6* 10 is the common allele in the Malaysian population. There is lack of data on local herbal-drug interaction. The objective of this study was to generate an in vitro drug-herbs int1eraction assay and use it to analyse local herb interaction. Recombinant CYP2D6* 1 was generated and co-expressed with CYPreductase in E. coli. Recombinant CYP2D6*10 was generated using site-directed mutagenesis and expressed as CYP2D6* 1.

Item Type:	Thesis (PhD)
Subjects:	R Medicine > RM Therapeutics. Pharmacology > RM300-666 Drugs and their actions
Divisions:	Kampus Kesihatan (Health Campus) > Pusat Pengajian Sains Perubatan (School of Medical Sciences)
Depositing User:	Mr Noorazilan Noordin
Date Deposited:	08 Sep 2017 00:56
Last Modified:	12 Apr 2019 05:26
URI:	http://eprints.usm.my/id/eprint/36415

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