Desaturase Delta-6 mRNA Expressions In Highly Unsaturated Fatty Acids Biosynthesis Pathway During Follicle Maturation In Female Zebrafish (Danio rerio)

Ishak, Sairatul Dahlianis (2010) Desaturase Delta-6 mRNA Expressions In Highly Unsaturated Fatty Acids Biosynthesis Pathway During Follicle Maturation In Female Zebrafish (Danio rerio). Masters thesis, Universiti Sains Malaysia.

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Abstract

Sangat sedikit yang diketahui tentang sintesis asid lemak tidak tepu seperti asid eikosapentanoik (EPA, C20:5n-3), asid dokosaheksaenoik acid (DHA, C22:6n- 3) dan asid arakidonik (ARA, C20:4n-6), atau secara berkumpulan lebih dikenali sebagai asid lemak sangat tidak tepu (HUF A), di dalam ovari ikan walaupun ia memainkan peranan yang sangat penting dalam pembiakan vertebrata ini. Ikan zebrafish (Dania rerio) mempamerkan keupayaan untuk mensintesis ketiga-tiga HUF A ini melalui kitaran yang melibatkan desaturasi dan elongasi dua prekursor iaitu asid linoleik (LA, Cl8:2n-6) dan asid linolenik (LNA, Cl8:3n-3). Bagi lebih memahami kepentingan serta pengawalaturan sintesis HUF A dalam ovari, kaj ian ini menunjukkan corak ekspresi mRNA untuk desaturase (jadsd6), iaitu satu enzim perlu yang terlibat dalam kitaran biosintesis HUF A dalam lima peringkat folikel ovari zebrafish yang berbeza iaitu pre-vitelogenik, vitelogenik awal, vitelogenik akhir, kematangan dan ovulasi. Aras mRNA bagi fadsd6 dalam peringkat-peringkat folikel ovari yang berbeza ini ditentukan oleh kaedah esei RT-PCR. Very little is known about the synthesis of unsaturated fatty acids such as eicosapentaenoic acid (EPA, C20:5n-3), docosahexaenoic acid (DHA, C22:6n-3) and arachidonic acid (ARA, C20:4n-6), or collectively known as the highly unsaturated fatty acids (HUF A), in the fish ovary although they play pivotal roles in this vertebrate reproduction. The zebrafish (Dania rerio) display capability to synthesize all three HUF A via pathways involving desaturation and elongation of two precursors, the linoleic acid (LA, C18:2n-6) and linolenic acid (LNA, Cl8:3n-3). In order to gain understanding on the importance and regulation of ovarian HUF A synthesis, this study shows the mRNA expression pattern of desaturase (jadsd6), an essential enzyme involved in HUF A biosynthesis pathway, in five different zebrafish ovarian follicle stages; pre-vitellogenic, early vitellogenic, late vitellogenic, maturation and ovulation stages. The mRNA levels of fadsd6 in different ovarian follicle stages were determined by semi-quantitative RT-PCR assays.

Item Type: Thesis (Masters)
Subjects: Q Science > QH Natural history > QH1 Natural history (General - Including nature conservation, geographical distribution)
Divisions: Pusat Pengajian Sains Kajihayat (School of Biological Sciences) > Thesis
Depositing User: Mr Noorazilan Noordin
Date Deposited: 09 Mar 2017 06:42
Last Modified: 12 Apr 2019 05:26
URI: http://eprints.usm.my/id/eprint/32377

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