Musa, Siti Fadhilah
(2016)
Synthesis And Characterisation Of Silver Nanoparticles Derived From Pleurotus sajor caju And Its Biological Activity On Candida albicans.
Masters thesis, Universiti Sains Malaysia.
Abstract
Nanopartikel perak (NP) telah dikaji secara meluas kerana keupayaannya sebagai anti-kulat dan anti-bakteria. Kaedah hijau untuk menghasilkan NP telah digunakan secara intensif kerana proses penghasilannya yang mesra persekitaran serta tidak menggunakan agen penurun yang toksik. C. albicans merupakan salah satu kulat yang boleh direncatkan pertumbuhannya oleh NP. C. albicans menjadi virulen kerana ia mempunyai keupayaan metabolik untuk hidup dalam kondisi pertumbuhan yang berbagai. C. albicans mempunyai kebolehan untuk hidup dalam kondisi ketiadaan atau kepekatan glukosa yang rendah. Bagi memastikan kelangsungan hidup, ia perlu meggunakan sumber karbon yang tidak fermentasi seperti asid laktik dan diproses melalui kitaran glioksilat manakala isositrat liase (CaICL1) merupakan gen yang terlibat dalam kitaran tersebut. Namun demikian, gen ini merupakan salah satu penyumbang kepada bahayanya C. albicans. Oleh itu, dalam kajian ini, NP yang terhasil dengan menggunakan ekstrak Pleurotus sajor caju atau dikenali sebagai cendawan tiram kelabu telah dikenalpasti setelah penukaran warna daripada kuning pucat ke oren kemerahan selepas 72 jam inkubasi.
Silver nanoparticle (AgNPs) is widely studied due to its antifungal and antimicrobial activities against a wide range of microorganisms. In previous study, C. albicans is one of the pathogenic yeast that has been reported to be inhibited by AgNPs. Green method used to synthesise AgNPs also has been intensively studied due to its environmentally friendly and the used of non-toxic reducing agent. Meanwhile C. albicans was found to be virulent due to its metabolic flexibility to survive in a wide range of growth conditions. It has the ability to grow in the absence or in low glucose concentration. In order for yeast to survive, it must assimilate the non-fermentable carbon sources such as lactate instead of glucose through glyoxylate cycle and the isocitrate lyase (CaICL1) is one of the genes involved in this cycle. Plus, the expression of the gene was reported to be one of the factors contributed to C. albicans virulence. In this study, AgNPs synthesised using Pleurotus sajor caju mushroom or its common name grey oyster mushroom from hot water extraction was used and it has been confirmed to form AgNPs by the color changed from pale yellow to reddish brown after 72 hours of incubation with 1mM AgNO3.
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