Molecular Dna Markers In Parentage Identification And Clonal Genetic Structure Of Cryptocoryne ×purpurea Ridl. nothovar. purpurea Hybrid Populations

Rusly, Rosazlina (2016) Molecular Dna Markers In Parentage Identification And Clonal Genetic Structure Of Cryptocoryne ×purpurea Ridl. nothovar. purpurea Hybrid Populations. PhD thesis, Universiti Sains Malaysia.

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Abstract

Penghibridan semulajadi telah diyakini kerapkali berlaku pada Cryptocoryne Wydler dan dianggap sebagai sumber kepada kerumitan taksonomi pada genus ini. Penyelidikan ini melibatkan gabungan kajian daripada data jujukan DNA (kawasan tertranskripsi dalaman (ITS) DNA nuklear ribosom dan gen matK daripada DNA kloroplas) untuk mengenalpasti induk kepada hibrid putatif Cryptocoryne daripada Semenanjung Malaysia. Berdasarkan kepada ciri-ciri morfologi tumbuhan ini dikenalpasti secara tentatif sebagai Cryptocoryne ×purpurea Ridl. nothovar. purpurea; tumbuhan steril yang telah lama dianggap sebagai hibrid, kemungkinan daripada dua spesies yang berkaitan; Cryptocoryne cordata Griff. var. cordata dan Cryptocoryne griffithii Schott. Status hibrid dan induk-induk ini dibuktikan secara bebas dengan kehadiran pada individu hibrid corak jujukan ITS daripada kedua-dua spesies induk ini. Tumbuhan hibrid ini berkongsi persamaan jujukan matK daripada C. cordata var. cordata dan C. griffithii, menunjukkan kedua-dua spesies induk putatif ini telah menjadi induk betina. Penghibridan timbal balik di antara kedua-dua spesies ini dilihat sebagai simetri dan bukan satu arah. Kajian ini juga bertujuan untuk membangunkan penanda mikrosatelit menggunakan jujukan generasi (Roche 454 pyrosequencing) daripada DNA genomik C. cordata var. cordata. Natural hybridization has been confirmed to occur frequently in Cryptocoryne Wydler and considered a source of taxonomic complexity in this genus. This research involved a combined study of DNA sequencing data (internal transcribed spacer (ITS) of nuclear ribosomal DNA and matK gene of chloroplast DNA) to identify the parentage of a putative Cryptocoryne hybrid from Peninsular Malaysia. Based on the morphological characters the plant was tentatively identified as Cryptocoryne ×purpurea Ridl. nothovar. purpurea; a sterile plant which has long been considered a hybrid, possibly from two related species; Cryptocoryne cordata Griff. var. cordata and Cryptocoryne griffithii Schott. The hybrid status and its putative parents was independently confirmed by the presence in hybrid individuals of an additive ITS sequence pattern from these two parental species. The hybrid plants shared the identical matK sequences from C. cordata var. cordata and C. griffithii, which indicated that both putative parental species had functioned as the maternal parent. Reciprocal hybridization between the two species seems to be symmetrical rather than unidirectional. This study also aimed at developing microsatellite markers using next generation sequencing (Roche 454 pyrosequencing) from the genomic DNA of C. cordata var. cordata.

Item Type:	Thesis (PhD)
Subjects:	Q Science > QH Natural history > QH1 Natural history (General - Including nature conservation, geographical distribution)
Divisions:	Pusat Pengajian Sains Kajihayat (School of Biological Sciences) > Thesis
Depositing User:	Mr Noorazilan Noordin
Date Deposited:	01 Mar 2017 09:09
Last Modified:	12 Apr 2019 05:25
URI:	http://eprints.usm.my/id/eprint/32253

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