Anamalai, Sanggari
(2016)
Isolation, Identification And Pcr Detection Of Methicillin Resistant Staphylococcus Aureus (Mrsa) In Cow Milk Samples From Perak.
Masters thesis, Universiti Sains Malaysia.
Abstract
Staphylococci mengkoloni haiwan dan manusia dan keadaan ini biasanya
tidak berbahaya. Walau bagaimanapun terdapat beberapa jenis Staphylococcus yang
mempunyai keupayaan untuk menyebabkan penyakit. Kaedah konvensional yang
diguna pakai sekarang iaitu berdasarkan ujian biokimia untuk mengenalpasti
staphylococci mengambil masa yang lama. Tambahan pula, kit yang diguna pakai
sekarang tidak dapat mengenalpasti perbezaan antara Staphylococcus koagulasenegatif
(CoNS). Sebagai alternatif, kaedah PCR amat berguna untuk mengenal pasti
strain patogen Staphylococcus. Dalam kajian ini sampel Staphylococcus telah
diasingkan daripada susu lembu dan dicirikan berdasarkan kaedah fenotipik.
Sebanyak dua ratus dua puluh lima pencilan dipencil dari empat pusat pengumpulan
iaitu Taiping, Tapah, Parit dan Sungai Siput telah diuji. Strain S. aureus telah diuji
berdasarkan hemolisis beta, pewarnaan Gram, ujian koagulase, ujian katalase, ujian
gula mannitol dan ujian gula maltosa. Sampel yang positif diuji dengan ujian
rintangan terhadap kesan antibiotik dengan menggunakan 1μg cakera oksasillin.
Keputusan ujian rintangan terhadap kesan antibiotik mengesahkan bahawa 19
pencilan S. aureus (12.41%) telah didapati menunjukkan ciri-ciri rintangan terhadap
ancaman antibiotik (MRSA), manakala 1 pencilan (0.65%) menunjukkan rintangan
pertengahan dan 133 (86.93%) sensitif terhadap kesan ancaman antibiotik (MSSA).
Staphylococci colonize a diverse range of animals and human and this
association is normally harmless. Some strains of Staphylococcus have increased
ability to cause disease. Biochemical tests used to identify staphylococci are lengthy.
Furthermore, commercially available panels do not allow a reliable distinction
between different coagulase-negative staphylococci (CNS). As an alternative, the
development of a PCR based technique will be useful to identify pathogenic strains
of Staphylococcus. In this study Staphylococcus samples were isolated from cow
milk and characterized using phenotypical methods. Two hundred and twenty five
isolates identified from four different collection centers in Taiping, Tapah, Parit and
Sungai Siput were tested. S. aureus strains were characterized by beta hemolysis,
Gram stain, coagulase test, catalase test, mannitol fermentation and maltose
fermentation. The positive samples were further investigated by antibiotic
susceptibility test by using 1μg oxacillin disc. Result of antibiotic susceptibility test
confirmed that 19 S.aureus isolates (12.41%) were found to be methicillin resistant
S. aureus (MRSA), while 1 isolate (0.65%) showed intermediate resistance and 133
(86.93%) as methicillin sensitive S. aureus (MSSA).
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