Phytochemical Studies Of Lippia Nodiflora (L.) Michx And Its Anti-Hyperuricemic Activity

Cheng, Lee Chuen (2016) Phytochemical Studies Of Lippia Nodiflora (L.) Michx And Its Anti-Hyperuricemic Activity. PhD thesis, Universiti Sains Malaysia.

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    Abstract

    Lippia nodiflora telah digunakan secara tradisional dalam Ayurveda, Unani, Sindh, dan Perubatan Tradisional Cina untuk rawatan sakit lutut sendi, lithiasis, diuresis, penyakit urinari dan bengkak. Dalam kajian ini, ekstrak metanol L. nodiflora menunjukkan aktiviti perencatan xantina oksidase (XOD) secara in vitro baik. Pemeringkatan berpandukan bioaktiviti ekstrak metanol menghasilkan empat pecahan (F1-F4) dengan F3 dikenal pasti sebagai pecahan yang paling poten. Penulenan F3 selanjutnya menghasilkan lima sebatian bioaktif, termasuk dua feniletanoid glikosida arenariosida (1) dan verbaskosida (2), dan tiga flavonoid 6-hidroksiluteolin (3), 6-hidroksiluteolin-7-O-glikosida (4), dan nodifloretin (5), di mana 1 dan 4 diasingkan untuk kali pertama daripada L. nodiflora. Ekstrak metanol, pecahan-pecahan, dan juzuk kimia tersebut kemudiannya diuji untuk activiti antihiperurisemik berpotensi secara in vivo dengan menggunakan model tikus hiperurisemik diaruh oleh kalium oxonate dan hipoksantina. Rawatan oral ekstrak metanol menurunkan paras asid urik serum tikus hiperurisemik dengan berkesan dan bersandarkan dos. Lippia nodiflora has been traditionally used in the Ayurvedic, Unani, Sindh, and Traditional Chinese Medicine for the treatment of knee joint pain, lithiasis, diuresis, urinary disorder and swelling. In the present study, methanol extract of L. nodiflora showed promising xanthine oxidase (XOD) inhibitory activity in vitro. Bioactivity-guided fractionation of methanol extract yielded four fractions (F1-F4) with F3 being identified as the most potent fraction. Further purification of F3 afforded five bioactive compounds, including two phenylethanoid glycosides arenarioside (1) and verbascoside (2), and three flavonoids 6-hydroxyluteolin (3), 6-hydroxyluteolin-7-O-glycoside (4), and nodifloretin (5), of which 1 and 4 were first time isolated from L. nodiflora. The methanol extract, fractions, and chemical constituents were then tested for potential antihyperuricemic activity in vivo using potassium oxonate- and hypoxanthine-induced hyperuricemic rat model. Oral treatment with methanol extract effectively and dose-dependently reduced the serum uric acid level of hyperuricemic rats.

    Item Type: Thesis (PhD)
    Subjects: R Medicine > RS Pharmacy and materia medica > RS1-441 Pharmacy and materia medica
    Divisions: Pusat Pengajian Sains Farmasi (School of Pharmacy) > Thesis
    Depositing User: Mr Noorazilan Noordin
    Date Deposited: 20 Feb 2017 12:13
    Last Modified: 22 Mar 2017 09:59
    URI: http://eprints.usm.my/id/eprint/32134

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