M.K Parthyban, Akash Parthi Bendran
(2016)
Antibacterial, Antioxidant And Platelet Boosting Properties Of Semalu And Manna Plus Extracts.
Masters thesis, Universiti Sains Malaysia.
Abstract
Semalu dan Manna Plus adalah produk komersial yang terkenal dengan nilai-nilai perubatannya seperti meningkatkan imuniti, antibiotik semula jadi yang sederhana potensinya, metabolisme protein dan mengimbangkan homeostasis. Para penyokong atau pengguna produk-produk ini mendakwa bahawa ia berkesan dalam meningkatkan platlet pesakit denggi. Kajian cytotoxicity, antioksidan, antibakteria dan peningkatan kadar platelet dijalankan untuk menilai sifat-sifat farmakologi ekstrak semalu dan manna plus. Kaedah HPTLC telah berjaya diwujudkan untuk menganalisis fitokimia eugenol, kurkumin, asid linoleik dan kuercetin untuk tujuan kuantifikasi dan kawalan mutu. Ekstrak terpiawai semalu dan manna plus telah digunakan untuk semua penilaian farmakologi. Keputusan sitotoksin manna plus, ekstrak metanol dan ethanol semalu menunjukkan tiada kesan toksik pada kepekatan <620 μg / mL dalam sel Chang HeLa. Merujuk kepada aktiviti antimikrob, semalu dan manna plus ekstrak etanol didapati aktif terhadap S. aureus, B. subtilis dan P. aeruginosa (MIC: 125-250 μg/mL) manakala ekstrak air menunjukkan perencatan terhadap bakteria gram positif sahaja (MIC: 250 μg / mL).
Semalu and Manna Plus are commercial products known for their medicinal values such as boosting immunity, natural mild antibiotic, protein metabolism and balancing homeostasis. The proponents or consumers of these products claim that they are efficient in boosting platelets in dengue acquired patients. In view of this some pharmacological properties of these extracts such as, cytotoxicity, antioxidant, antibacterial and platelet boosting effect were undertaken. HPTLC methods were successfully developed for phytochemical analysis of eugenol, curcumin, linoleic acid and quercetin respectively for quantification and quality control purposes. Standardized extracts of semalu and manna plus were employed for all pharmacological evaluations. The cytotoxicity results demonstrated manna plus and semalu methanolic and ethanolic extracts exhibited nontoxic effects at <620 μg/mL on Chang HeLa cells. With regards to antimicrobial activity, semalu and manna plus ethanolic extracts were active against S. aureus, B. subtilis and P. aeruginosa (MIC: 125-250 μg/mL). However water extracts showed inhibition only against gram positive (MIC: 250 μg/mL) bacteria.
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