Perumal, Shanmugapriya
(2015)
Antibacterial Activity Of Euphorbia Hirta
(L.): Euphorbiaceae And Isolation Of Its
Bioactive Constituents.
PhD thesis, Universiti Sains Malaysia.
Abstract
Produk semulajadi, terutamanya tumbuhan ubatan, kekal sebagai sumber
berharga sebagai pendekatan revolusi hijau dalam penemuan sebatian antibakteria
novel. Tumbuhan Euphorbia hirta (L.) digunakan secara tradisional di negara-negara
tropika untuk rawatan gastrousus, bronkial dan penyakit pernafasan yang disebabkan
oleh agen jangkitan nosokomial. Dalam kajian ini, pelbagai ekstrak pelarut (metanol,
etanol, etil asetat, diklorometana dan heksana) daripada tumbuhan E. hirta telah dikaji
dalam penyaringan fitokimia untuk menentukan jumlah kandungan fenolik dan
flavonoid. Aktiviti antibakteria yang berpotensi daripada ekstrak mentah dinilai
melalui ujian in vitro antibakteria dengan menggunakan kaedah mikropencairan
medium brot terhadap panel patogen klinikal yang rintang dan strain jenis kultur
koleksi Amerika (ATCC). Ekstrak yang paling bioaktif, ekstrak metanol,
kemudiannya menjalani penulenan dengan kaedah fraksinasi dan pemencilan
berdasarkan bioaktiviti menggunakan kromatografi turus, yang akhirnya menghasilkan
tiga sebatian tulen antibakteria.
Natural products, especially medicinal plants, remain as a valuable source
offering a revolutionary green approach to the discovery of novel antibacterial
compounds. Euphorbia hirta (L.) plant is traditionally used in many tropical countries
for the treatment of gastrointestinal, bronchial and respiratory ailments caused by
nosocomial infectious agents. In this study, various solvent extracts (methanol,
ethanol, ethyl acetate, dichloromethane and hexane) of E. hirta were subjected to
phytochemical screening to determine total phenolic and flavonoid contents. The
potential antibacterial activities of the crude extracts were assessed via in vitro
antibacterial test using broth microdilution method against a panel of clinically
resistant pathogens and American Type Culture Collection (ATCC) strains. The most
bioactive extract, the methanol extract was further purified in a bioactivity-guided
fractionation and isolation method using column chromatography, which finally
yielded three pure antibacterial compounds.
Actions (login required)
 |
View Item |
![[img]](http://eprints.usm.my/style/images/fileicons/application_pdf.png)
