Standardization, Safety And Antiobesity Studies Of Piper Sarmentosum Roxburgh Extracts

Hamil, Mohd Shahrul Ridzuan (2016) Standardization, Safety And Antiobesity Studies Of Piper Sarmentosum Roxburgh Extracts. Masters thesis, Universiti Sains Malaysia.

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    Abstract

    Kajian ini merangkumi kawalan kualiti, pemiawaian, keselamatan dan aktiviti biologi menggunakan ekstrak Piper sarmentosum. Ekstrak menggunakan etanol, etanol (50%) dan air telah dipiawai dan dikaji menggunakan assai anti-obesiti dan antihiperlipidemik. Ujian fiziko-kimia, had, cap jari kualitatif dan kuantitatif dijalankan menggunakan kaedah spektroskopi dan kromatografi. Kandungan metabolit primer dan sekunder dalam ekstrak ditentukan. Ekstrak telah dianalisis bagi kandungan asaron menggunakan HPLC dan FT-IR. Analisis kemometri (PCA dan HCA) diaplikasikan ke dalam pemiawaianan. Ekstrak terpiawai disaring secara in vitro menggunakan assai sitotoksisiti udang brin (BSLA), perencatan lipase pankreas (PPL) dan aktiviti antiangiogenesis. Assai anti-obesiti dan anti-hiperlipidemik dijalankan secara in vivo dalam tikus teraruh diet tinggi lemak (HFD). This study involves quality control, standardization, safety and biological activities using Piper sarmentosum extracts. Extracts using ethanol, ethanol (50%) and water were standardized and further evaluated using anti-obesity and antihyperlipidemic assays. Physico-chemical, limit test, qualitative and quantitative plant fingerprint was performed using spectroscopic and chromatographic techniques. The contents of primary and secondary metabolites in the extracts were determined. The extracts were analyzed for asarones content using HPLC and FT-IR. Chemometrics (PCA and HCA) were applied in standardization. Standardized extracts were screened in vitro for cytotoxicity against brine shrimp assay (BSLA), pancreatic lipase (PPL) inhibition and anti-angiogenesis activities. The anti-obesity and anti-hyperlipidemic assays were performed in vivo on high fat diet (HFD) induced rats.

    Item Type: Thesis (Masters)
    Subjects: R Medicine > RS Pharmacy and materia medica > RS1-441 Pharmacy and materia medica
    Divisions: Pusat Pengajian Sains Farmasi (School of Pharmacy) > Thesis
    Depositing User: Mr Noorazilan Noordin
    Date Deposited: 26 Jan 2017 12:55
    Last Modified: 22 Mar 2017 09:59
    URI: http://eprints.usm.my/id/eprint/31836

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