Hamil, Mohd Shahrul Ridzuan
(2016)
Standardization, Safety And Antiobesity Studies Of Piper Sarmentosum Roxburgh Extracts.
Masters thesis, Universiti Sains Malaysia.
Abstract
Kajian ini merangkumi kawalan kualiti, pemiawaian, keselamatan dan aktiviti
biologi menggunakan ekstrak Piper sarmentosum. Ekstrak menggunakan etanol, etanol
(50%) dan air telah dipiawai dan dikaji menggunakan assai anti-obesiti dan antihiperlipidemik.
Ujian fiziko-kimia, had, cap jari kualitatif dan kuantitatif dijalankan
menggunakan kaedah spektroskopi dan kromatografi. Kandungan metabolit primer dan
sekunder dalam ekstrak ditentukan. Ekstrak telah dianalisis bagi kandungan asaron
menggunakan HPLC dan FT-IR. Analisis kemometri (PCA dan HCA) diaplikasikan ke
dalam pemiawaianan. Ekstrak terpiawai disaring secara in vitro menggunakan assai
sitotoksisiti udang brin (BSLA), perencatan lipase pankreas (PPL) dan aktiviti antiangiogenesis.
Assai anti-obesiti dan anti-hiperlipidemik dijalankan secara in vivo dalam
tikus teraruh diet tinggi lemak (HFD).
This study involves quality control, standardization, safety and biological
activities using Piper sarmentosum extracts. Extracts using ethanol, ethanol (50%) and
water were standardized and further evaluated using anti-obesity and antihyperlipidemic
assays. Physico-chemical, limit test, qualitative and quantitative plant
fingerprint was performed using spectroscopic and chromatographic techniques. The
contents of primary and secondary metabolites in the extracts were determined. The
extracts were analyzed for asarones content using HPLC and FT-IR. Chemometrics
(PCA and HCA) were applied in standardization. Standardized extracts were screened in
vitro for cytotoxicity against brine shrimp assay (BSLA), pancreatic lipase (PPL)
inhibition and anti-angiogenesis activities. The anti-obesity and anti-hyperlipidemic
assays were performed in vivo on high fat diet (HFD) induced rats.
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