Expression, Purification And Crystallization Trials Of Small Rubber Particle Protein (Srpp) From Hevea Brasiliensis

Satinder Singh, Saranpal Singh (2016) Expression, Purification And Crystallization Trials Of Small Rubber Particle Protein (Srpp) From Hevea Brasiliensis. Masters thesis, Universiti Sains Malaysia.

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    Abstract

    Getah asli daripada Hevea brasiliensis yang diperbuat daripada cis-1,4-poliisopren, merupakan komoditi perusahaan yang penuh dengan ciri-ciri keunikan. Biosintesis getah asli daripada H. brasiliensis berlaku pada permukaan partikel getah yang dimangkinkan oleh ‘small rubber particle protein’ (SRPP) dan ‘rubber elongation factor’ (REF). Protein SRPP ini dipercayai menggabungkan isopentenil difosfat (IPP) pada permukaan partikel getah. Tambahan pula, protein SRPP juga merupakan salah satu alergen getah utama dalam H. brasiliensis. Walaupun terdapat banyak bukti yang menunjukkan penglibatan protein ini dalam biosintesis getah dan alergen, namun, masih terdapat kekurangan penyelidikan yang mengaitkan mekanisme SRPP dalam sintesis getah dan mekanisme alahan. Natural rubber (NR) of Hevea brasiliensis predominantly made up of cis-1,4-polyisoprene, is an essential industrial commodity with unique characteristics. The biosynthesis of NR from H. brasiliensis is catalyzed on the surface of rubber particles by a set of integrated proteins, namely the small rubber particle protein (SRPP), and rubber elongation factor (REF). The SRPP protein has been speculated to incorporate isopentenyl diphosphate (IPP) monomers in rubber particles. Furthermore, the SRPP protein has also been implicated as a major latex allergen.

    Item Type: Thesis (Masters)
    Subjects: Q Science > QH Natural history > QH1 Natural history (General - Including nature conservation, geographical distribution)
    Divisions: Pusat Pengajian Sains Kajihayat (School of Biological Sciences) > Thesis
    Depositing User: Mr Noorazilan Noordin
    Date Deposited: 25 Jan 2017 14:10
    Last Modified: 10 Apr 2017 10:12
    URI: http://eprints.usm.my/id/eprint/31810

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