Development Of Novel Exosome-Based Delivery System For Proteins

Chew , Yik Wei (2016) Development Of Novel Exosome-Based Delivery System For Proteins. Masters thesis, Universiti Sains Malaysia.

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    Abstract

    Sel mempunyai beribu-ribu protein yang menyumbang kepada laluan isyarat penting terhadap fungsi sel normal. Kebanyakan penyakit terjadi akibat daripada kepincangan tugas salah satu atau lebih daripada protein berkenaan. Untuk meredakan disfungsi berkenaan, terapi protein yang menggunakan protein terapeutik untuk membetulkan fungsi sel dianggap pendekatan yang paling selamat untuk merawat penyakit. Terdapat lebih daripada 1,500 sasaran rawatan penyakit yang berpotensi di dalam sel tanpa laluan reseptor yang jelas sebagai sasaran khusus mereka. Keperluan untuk penghantaran intra sel yang mensasarkan sel perumah tertentu merupakan satu halangan yang sukar dalam pembangunan intra sel baharu yang berasaskan protein. Exosomes yang bersaiz 40-120nm, merupakan vesikel membran yang dirembeskan oleh sel dan telah dikenalpasti sebagai pengangkutan semulajadi untuk penghantaran makromolekul. Exosomes berinteraksi dengan sel dan turut memberi kesan terhadap fungsi sel lain yang berdekatan melalui pemindahan inter sel mRNA, microRNA, reseptor dan enzim, dan didapati memainkan peranan penting dalam kawalselia imun. A cell contains thousands of proteins that contribute to critical signalling pathways in normal cellular functions. Most diseases somehow result from the malfunction of one or more of such proteins. To palliate such dysfunction, protein therapy that makes use of therapeutic proteins to correct the cellular functions is considered the most direct and safe approach for treating diseases. There are more than 1,500 potential disease treatment targets that reside inside the cell, without a clear receptor pathway for their specific targeting. The need for intracellular delivery and targeting select host cell compartment represents a formidable hurdle to the development of novel prospective intracellular targeting protein-based biologics. Exosomes, 40-120 nm membrane vesicles secreted by cells, have recently emerged as promising natural vehicles for the delivery of macromolecules. Exosomes interact with cells and affect the functions of neighbouring cells through intercellular transfer of mRNA, microRNA, receptors and enzymes, and found to play important roles in immune dysregulation.

    Item Type: Thesis (Masters)
    Subjects: R Medicine > RK Dentistry > RK1-715 Dentistry
    Divisions: Institut Perubatan & Pergigian Termaju (Advanced Medical & Dental Institute) > Thesis
    Depositing User: Mr Noorazilan Noordin
    Date Deposited: 24 Jan 2017 10:44
    Last Modified: 24 May 2017 09:15
    URI: http://eprints.usm.my/id/eprint/31774

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