Heterologous Expression Of Avian Influenza A (H5n1) Neuraminidase In Kluyveromyces Lactis And Escherichia Coli

Taufik, Noor Zafiah (2016) Heterologous Expression Of Avian Influenza A (H5n1) Neuraminidase In Kluyveromyces Lactis And Escherichia Coli. Masters thesis, Universiti Sains Malaysia.

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Abstract

Pengekspresan neuraminidase (NA) virus H5N1 di dalam sistem pengekspresan mikrob yang ringkas dengan menggunakan Kluyveromyces lactis dan Escherichia coli sebagai perumah telah dikaji. Untuk penghasilan protein NA dalam K. lactis, dua jenis fragmen DNA NA terdiri daripada 1) jujukan penuh (1347 bp) dan 2) jujukan domain kepala (residu 63-449, 1161 bp) NA daripada A/Chicken/Malaysia/5858/2004 (H5N1) telah diamplikasi dan diklon ke dalam vektor pengekspresan pKLAC2 lalu menghasilkan konstruk plasmid yang dinamakan pKLAC2-NA and pKLAC2-NAHD. The expression of H5N1 virus neuraminidase (NA) in simple microbial expression system utilizing Kluyveromyces lactis and Escherichia coli as the host was studied. For NA protein production in K. lactis, two types of NA DNA fragment composed of 1) full length sequence (1347 bp) and 2) head domain sequence (residue 63-449, 1161 bp) from A/Chicken/Malaysia/5858/2004 (H5N1) were amplified and cloned into pKLAC2 expression vector generating plasmid constructs named pKLAC2-NA and pKLAC2-NAHD.

Item Type:	Thesis (Masters)
Subjects:	Q Science > QH Natural history > QH1 Natural history (General - Including nature conservation, geographical distribution)
Divisions:	Pusat Pengajian Sains Kajihayat (School of Biological Sciences) > Thesis
Depositing User:	Mr Firdaus Mohamad
Date Deposited:	17 Jan 2017 04:21
Last Modified:	12 Apr 2019 05:25
URI:	http://eprints.usm.my/id/eprint/31644

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