In Vitro Antibacterial And Wound Healing Properties Of A Standardized Polyphenols-Rich Fraction Of Dicranopteris Linearis (BURM.) Underwood

Ponnusamy, Yasodha (2016) In Vitro Antibacterial And Wound Healing Properties Of A Standardized Polyphenols-Rich Fraction Of Dicranopteris Linearis (BURM.) Underwood. PhD thesis, Universiti Sains Malaysia.

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    Abstract

    Dicranopteris linearis (Burm.) Underwood merupakan sejenis paku-pakis menjalar tanah yang digunakan secara tradisional untuk merawat pelbagai penyakit kulit seperti bisul, ulser dan luka luaran. Sehingga kini, tiada kajian saintifik yang dijalankan secara sistematik untuk menilai kegunaan etnofarmakologikal D. linearis untuk mempercepatkan penyembuhan luka.Dalam kajian ini, ekstrak MeOH daripada D. linearis telah diperkaya dengan sebatian bioaktif dengan menggunakan kaedah berpandukan bioaktiviti. Salah satu fraksi ekstrak (F5) yang diperoleh telah didapati memiliki aktiviti antioksidan dan aktiviti antibakteria yang signifikan terhadap S. aureus dan P. aeruginosa. Analisis telah dilanjutkan untuk mengenal pasti kompoun dalam fraksi aktif ini. Pemencilan sebatian telah dijalankan dengan menggunakan gabungan teknik kromatokrafi.gel silica fasa terbalik dan normal. Dicranopteris linearis (Burm.)Underwood is a ground creeping fern used traditionally to treat various skin related ailments, such as boils, ulcers and external wounds. To date, no scientific studies have been carried out to evaluate its ethnopharmacological use in wound infection in a systematic manner. In the present study, the MeOH extract of D. linearis was fractionated with the bioactive constituents following a bioactivity-guided approach. A fraction (F5) of the extract was obtained and it was found to possess significant antioxidant and antibacterial activity against S. aureus and P. aeruginosa. Analysis was further carried out to identify the chemical constituents of this active fraction. Isolation of the major constituents was carried out using a combination of reversed-phase and normal-phase silica gel chromatographic techniques.

    Item Type: Thesis (PhD)
    Subjects: R Medicine > RM Therapeutics. Pharmacology > RM300-666 Drugs and their actions
    Divisions: Pusat Penyelidikan Dadah dan Ubat-ubatan (Centre for Drug Research)
    Depositing User: Mr Noorazilan Noordin
    Date Deposited: 16 Jan 2017 09:12
    Last Modified: 16 Jan 2017 09:23
    URI: http://eprints.usm.my/id/eprint/31589

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