Effects Of Selected Additives For Growth Efficiency On In Vitro Grown Vanilla Planifolia Andrews

Goh, Foong Jing (2016) Effects Of Selected Additives For Growth Efficiency On In Vitro Grown Vanilla Planifolia Andrews. Masters thesis, Universiti Sains Malaysia.

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Abstract

Vanilla planifolia Andrews ialah ramuan makanan yang mempunyai nilai ekonomi dan kedua paling mahal di dunia. Dalam kajian yang lepas, bahan tambahan organik telah menujuk potensi mengingkatkan perkembangan dan pertumbuhan pokok tumbuhan tetapi kajian yang teliti terhadap V. planifolia terhad. Maka, dalam kajian ini, kesan bahan tambahan organik berbeza yang dipilih telah dikaji terhadap pertumbuhan dan perkembangan V. planifolia Andrews. Peningkatan protokol yang lebih baik untuk pertumbuhan tumbuhan dan perkembangan vanila telah dijalankan dan akhirnya mengkaji perbezaan morfologi anak pokok yang ditanam secara in vitro dan ex vitro. Pucuk V. planifolia dikultur dalam kekuatan medium MS yang berbeza, kepekatan BAP dan sukrosa yang berbeza, dan jenis ejen gel yang berlainan untuk mengkaji medium pertumbuhan yang terbaik. Pertumbuhan anak pokok V. planifolia direkodkan selepas dirawat selama lapan minggu. Hasil Kajian menunjukkan pembekalan ½ kekuatan medium MS dan 20 g/L sukrosa didapati cukup berkesan untuk menggalakkan pertumbuhan pokok. Vanilla planifolia Andrews is the economically important and second most expensive food ingredients in the worldwide. In previous studies, organic additives have shown their potential on promoting plant growth and development, however the detail studies of organic additive on plant growth and development of V. planifolia was limited. Thus, in this research, the effects of different selected organic additives were studied on the plant growth and development of V. planifolia Andrews. Improvement on the protocol for plant growth and proliferation of vanilla were carried out and finally to study the morphological differences between the in vitro and ex vitro plantlets. V. planifolia plant shoots were initially cultured on different strengths of MS, different concentrations of BAP, sucrose and different types of gelling to determine the best growth medium. The V. planifolia plantlets growth were recorded after eight weeks of treatment. Supplemented of ½ strength MS medium and supplementation of 20 g/L were efficient enough to promoted plant growth.

Item Type:	Thesis (Masters)
Subjects:	Q Science > QH Natural history > QH1 Natural history (General - Including nature conservation, geographical distribution)
Divisions:	Pusat Pengajian Sains Kajihayat (School of Biological Sciences) > Thesis
Depositing User:	Mr Noorazilan Noordin
Date Deposited:	12 Jan 2017 06:16
Last Modified:	12 Apr 2019 05:25
URI:	http://eprints.usm.my/id/eprint/31559

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