Effects Of Galactose And Fructose On The Expression Of Isocitrate Lyase (Icl1) Enzyme In Candida Albicans

Ting , Seng Yeat (2016) Effects Of Galactose And Fructose On The Expression Of Isocitrate Lyase (Icl1) Enzyme In Candida Albicans. Masters thesis, Universiti Sains Malaysia.

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    Abstract

    Sifat kepatogenan dalam C. albicans bergantung kepada atribut kecergasan dan juga faktor-faktor virulen yang lain. Ini termasuk sifat kental terhadap tindak balas tekanan dan penyesuaian metabolik. C. albicans boleh menduduki kebanyakkan persekitaran dalam ruang badan manusia yang mengandungi kepelbagaian sumber karbon yang berbeza. Asimilasi sumber karbon adalah penting untuk pertumbuhan dan juga untuk meneruskan jangkitan. Tesis ini mengkaji kesan galaktosa atau fruktosa terhadap asimilasi sumber karbon sekunder seperti laktat oleh C. albicans. Gen C. albicans Isositrat liase (CaICL1) ditindas oleh penambahan 2% galaktosa atau fruktosa kepada sel-sel yang sedang membiak pada laktat. Gen CaICL1 yang mengekod kitaran glioksilat enzim Icl1 diperlukan untuk pertumbuhan dalam mengunakan sumber karbon sekunder seperti laktat. Enzim CaIcl1 didapati stabil dalam galaktosa tetapi tidak stabil dalam fruktosa. Sebaliknya, kedua-dua galaktosa dan fruktosa mendegradasikan protein S. cerevisiae Icl1 (ScIcl1). The virulence of C. albicans is dependent upon fitness attributes as well as virulence factors. These attributes include robust stress responses and metabolic flexibility. C. albicans can occupy a variety niches in human, many of which contain a range of different carbon sources. The assimilation of these carbon sources is important for growth and essential for establishment of infections by C. albicans. This thesis examines the impact of galactose or fructose upon the assimilation of secondary carbon sources such as lactate by C. albicans Isocitrate lyase gene (CaICL1) is repressed upon addition of 2% galactose or fructose to lactate- grown cells. The CaICL1 gene, which encode the glyoxylate cycles enzymes isocitrate lyase are required for growth on non- fermentable carbon sources such as lactate. However the enzyme CaIcl1 was not destabilized by galactose, but was degraded in response to fructose. In contrast, S. cerevisiae Icl1 (ScIcl1) was rapidly degraded in response to either galactose or fructose.

    Item Type: Thesis (Masters)
    Subjects: R Medicine > RK Dentistry > RK1-715 Dentistry
    Divisions: Institut Perubatan & Pergigian Termaju (Advanced Medical & Dental Institute) > Thesis
    Depositing User: Mr Noorazilan Noordin
    Date Deposited: 10 Jan 2017 12:37
    Last Modified: 24 May 2017 09:15
    URI: http://eprints.usm.my/id/eprint/31499

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