Johan, Fairooz
(2016)
Chlorophyll A Concentration Of Fresh Water Phytoplankton Analysed By Algorithmic Based Spectroscopy.
PhD thesis, Universiti Sains Malaysia.
Abstract
Fitoplankton ialah tumbuhan microskopik sel tunggal yang memainkan
peranan penting di dalam ekosistem sebagai pengeluar primari utama melalui aktiviti
fotosintesis. Objektif utama kajian ini ialah untuk mengkaji pencirian melalui
hubungan pantulan dan kepekatan klorofil a fitoplankton di dalam air tawar. Melalui,
kajian ini, pantulan fitoplankton diambil dengan menggunakan spektroradiometer
untuk melihat hubungan antara pantulan dan panjang gelombang fitoplankton di
dalam air tawar. Satu lokasi tasik dalam Universiti Sains Malaysia yang dikenali
sebagai Tasik Harapan dipilih untuk kajian ini. Sebanyak 20 liter sampel air diambil
dan ditapis dengan menggunakan jaring fitoplankton. Sampel air yang diambil
dianalisis di dalam makmal untuk menentukan pantulan dan kandungan kepekatan
klorofil-a fitoplankton. Dua jenis spektrometer digunakan di dalam kajian ini
pertama, spektroradiometer digunakan untuk mengukur pantulan fitoplankton dan
kedua, spektrofotometer digunakan untuk mengukur kepekatan klorofi a. Sampel air
yang diambil dikultur terlebih dahulu dengan memasukkan dalam medium yang
dikenali sebagai Bold's Basal sebelum nilai pantulan diambil agar pengukuran
pantulan tepat ke atas fitoplankton.
Phytoplankton are microscopic single-celled plants that play an important
role in the ecosystem as a major primary producers through photosynthesis. The
main objective of this study was to investigate properties through reflection and
chlorophyll a concentrations of phytoplankton in freshwater. In this study, reflection
phytoplankton were taken using spectroradiometer to observe the relationship
between the reflectance and the wavelengths of phytoplankton in freshwater. The
lake selected for this study is known as Tasik Harapan located in Universiti Sains
Malaysia. In this study, 20 litres water samples undergone the filtering using
phytoplankton net. The water samples collected were analyzed in the laboratory to
determine the reflectance and chlorophyll a concentration of phytoplankton. Two
spectrometers were used in this study, firstly, spectroradiometer which was used to
measure the reflectance of phytoplankton and secondly, spectrophotometer which
was used to measure the concentration of chlorophyll a. The water samples taken
were prior to culture in the medium known as Bold's Basal Medium before the
reflections were taken so that accurate reflection measurements on phytoplankton
can be acquired.
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