Zakaria, Robaiza
(2008)
Experimental Rnomics: Towards The
Identification And Characterization Of
Non-Protein-Coding Ribonucleic Acids In
Pathogenic Agent, Salmonella Typhi.
PhD thesis, Universiti Sains Malaysia.
Abstract
RNA bukan-pengkod-protein (npcRNA) merupakan satu kelas pengawalatur-ribo
yang bertindak di dalam bentuk kompleks RNA-protein (sebagai RNPs) didalam
pelbagai laluan pengawalaturan. Tesis ini memberikan tumpuan ke atas_ pengenalpastian
npcRNA secara eksperimental daripada bakteria patogenik Salmonella enterica serovar
Typhi (S. Typhi), penyebab penyakit demam kepialu. Melalui pendekatan RNomiks
Eksperimental, 82 calon novel npcRNAdaripada perpustakaan cDNA S. Typhi telah
dikenalpasti dan dicirikan. Daripada jumlah ini, 28 telah ditranskrip daripada IGR, 29
ditranskrip di dalam arah antisense kepada ORF dan 18 dikenalpasti bertindihan dengan
ORF. Sementara 7 calon yang lain telah ditranskIlp daripada kawasan repititif dan
beberapa kedudukan bukan repitatif yang lain. Sebelas npcRNA merupakan npcRNAs
yang telahpun dilaporkan.
Non-protein-coding RNA (npcRNA) is a large class of riboregulators that act in
complex with proteins (as RNPs) in diverse regulatory pathways. This thesis focused on
the experimental identification of small npcRNAs from Salmonella enterica serovar
Typhi (s. Typhi), the aetiological agent of typhoid fever. By an Experimental RNomics
approach, 82 species of uncharacterized novel npcRNA candidates were identified from
library generated from different growth phases of a clinically isolated S. Typhi. From
this, 28 were transcribed from the IGRs, 29 were transcribed in the antisense orientation
of the ORFs and 18 were identified to overlap the ORFs. Another 7 candidates were
transcribed from repetitive regions and several non-repetitive locations. Eleven known
npcRNAs were also detected.
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