Lipase Termotoleran Daripada Geobacillus Thermodenitrificans Dan Penggunaannya Di Dalam Tindak Balas Esterifikasi Dan Enantiomerik

Him, Nik Raikhan Nik (2010) Lipase Termotoleran Daripada Geobacillus Thermodenitrificans Dan Penggunaannya Di Dalam Tindak Balas Esterifikasi Dan Enantiomerik. PhD thesis, Universiti Sains Malaysia.

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Abstract

Circular airlift fermenter system (FAUK) was found to be the best system in producing thermotolerant lipase enzyme from Geobacillus thermodenitrificans (LGT). The results revealed that optimum parameters were, temperature of 65°C, pH of 6.8 and inoculum size at 7.0% (v/v); which were used in the kinetic studies of FAUK system using production medium containing 0.75% (w/v) glucose, 1.25% (w/v) yeast extract, 0.45% (w/v) NaCl and 0.10% (v/v) olive oil (Nik Raikhan, 2003). Glucose at 1.25% (w/v) and air flow rate of 1.00 L/min were the best parameters. Kinetic approach was focused on the effect of glucose concentrations and air flow rates to the cell growth and the production of LGT. The results revealed that specific growth, μ was highest with 1.25% (w/v) and flow rate of 1.0 L/min which was 0.12 hour-1. This cultivation has the lowest doubling time, td that was 5.77 hours, the rate of enzyme formation, dp/dt of 1.73 Uml-1h- 1, a very high rate of specific enzyme production which was recorded as 11700.0 U/g of mass, the rate of glucose used, ds/dt of 0.22 gL-1h-1 and quite a low formation of enzyme per gram, that was 4110.0 U/g of glucose.

Item Type: Thesis (PhD)
Subjects: Q Science > QH Natural history > QH1 Natural history (General - Including nature conservation, geographical distribution)
Divisions: Pusat Pengajian Sains Kajihayat (School of Biological Sciences) > Thesis
Depositing User: Mr Mohammad Harish Sabri
Date Deposited: 08 Oct 2018 03:01
Last Modified: 12 Apr 2019 05:26
URI: http://eprints.usm.my/id/eprint/42346

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