Phylogenetics Of Mud Crab Genus Scylla And Phylogeographical Distribution Of S. Olivacea (Brachyura: Portunidae) In Malaysia

Mohamad Rosly, Hurul Adila-Aida (2016) Phylogenetics Of Mud Crab Genus Scylla And Phylogeographical Distribution Of S. Olivacea (Brachyura: Portunidae) In Malaysia. Masters thesis, Universiti Sains Malaysia.

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    Abstract

    Kajian ini tertumpu kepada filogenetik genus Scylla dan taburan filogeografi S. olivacea di Malaysia berdasarkan 542 pasangan bes (bp) gen mitokondria sitokrom c oksidase I (COI). Sebanyak 201 individu telah disampel dari tujuh lokasi di seluruh Semenanjung Malaysia, dan satu lokasi dari Sabah dan Sarawak. Spesies yang paling banyak ialah S. olivacea (111 individu), diikuti oleh S. tranquebarica (61 individu) dan S. paramamosain (29 individu). Namun begitu, spesimen S. serrata tidak dijumpai semasa persampelan dan sampel import telah diperolehi dari restoran untuk dimasukkan ke dalam analisis. Analisis filogenetik dijalankan dengan menggunakan kaedah “neighbour-joining” (NJ) dan “maximum parsimony” (MP) berdasarkan perisian MEGA versi 5.05. Kedua-dua kaedah statistik mendedahkan hubungan monofiletik antara S. olivacea, S. paramamosain, S. tranquebarica dan S. serrata dan menyokong kuat kehadiran tiga spesies Scylla iaitu S. olivacea, S. paramamosain, S. tranquebarica dan ketiadaan S . serrata di perairan Malaysia. Jujukan haplotip telah diperolehi dengan menggunakan perisian Collapse versi 1.2, yang mendedahkan 66 jujukan haplotip S. olivacea; di mana 14 daripada mereka berkongsi jujukan yang sama antara populasi manakala jujukan selebihnya adalah unik. Analisis varian molekul (AMOVA) telah dijalankan menggunakan perisian ARLEQUIN versi 3.11. This study was focused on the phylogenetics of the genus Scylla and phylogeographical distribution of S. olivacea in Malaysia based on 542 base pairs (bp) of the mitochondrial cytochrome c oxidase I (COI) gene. A total of 201 individuals were sampled from seven locations throughout peninsular Malaysia, and a single population each from Sabah and Sarawak. The most abundant species was S. olivacea (111 individuals), followed by S. tranquebarica (61 individuals) and S. paramamosain (29 individuals). However, S. serrata specimens were not found during sampling and therefore imported samples were obtained from restaurants for inclusion in the analyses. Phylogenetic analyses were conducted using the neighbour – joining (NJ) and maximum parsimony (MP) methods based on MEGA ver. 5.05. Both statistical methods revealed the reciprocally monophyletic relationships among S. olivacea, S. paramamosain, S. tranquebarica and S. serrata strongly supporting the presence of three species of Scylla namely S. olivacea, S. paramamosain, S. tranquebarica and the absence of S. serrata in Malaysian waters. Haplotype sequences were obtained using Collapse ver. 1.2, revealing 66 haplotype sequences of S. olivacea; where 14 of them were shared among populations while the remaining sequences were unique. Analysis of molecular variance (AMOVA) was conducted using ARLEQUIN ver. 3.11 software.

    Item Type: Thesis (Masters)
    Subjects: Q Science > QH Natural history > QH1 Natural history (General - Including nature conservation, geographical distribution)
    Divisions: Pusat Pengajian Sains Kajihayat (School of Biological Sciences) > Thesis
    Depositing User: Mr Noorazilan Noordin
    Date Deposited: 14 Feb 2017 15:41
    Last Modified: 10 Apr 2017 10:12
    URI: http://eprints.usm.my/id/eprint/32073

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